Reprotox Biotech-In vitro toxicity testing

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Animal free murine 3D-M®Repro

ReproTox has developed an animal-free 3D testicular cell co-culture system (3D-T®Repro) from a combination of testicular cell lines. This model has gone through extensive biomarker and morphology testing to ensure that it reflects In vitro condition and provides affordable, humane, and efficient methods for reproductive and developmental toxicity screening.

3D-T®Repro_Primary

ReproTox has developed a proprietary technology to establish in vitro 3D testicular model at different stage of spermatogenesis from mice, rats and canine. We derived testicular cells from neonate, juvenile and adolescent, established these testicular-like organoid representing differential stages of spermatogenesis. This organoid culture recapitulate many features of spermatogenesis in vivo, and enable assessment of differentiation and mitosis. This technology provides a reliable, robust, and relevant in vitro system for preclinical drug discovery, repro-toxicity assessment, and disease modeling.An extensive panel of toxicological endpoints is assessed in multiple cell types and at multiple time points using High-Content screening (HCS). By combining these endpoints with exposure levels, the system delivers a sensitive and specific prediction of repro-toxicity.

3D-T®Repro_EX-Vivo

3D-T®Repro_EX-Vivo provides an innovative technology to culture seminiferous tubes, enable the spermatogenesis In vitro. The technology preserves the original structure of the seminiferous tubules, and the Sertoli cells remain polarized which is crucial for the differentiation of germ cells. Thus, it allows the study of both spermatogenesis in mitotic and meiotic phases.

High-Content BioAssay System

ReproTox is at the forefront of cell-based High- Throughput (HT) and pathway-based High- Content Analysis (HCA) for reproductive-toxicity evaluations. This means we can provide a fully automated high-throughput screen and high- content analysis as well as access to the reproductive toxicity database developed by ReproTox (150 compounds). ReproTox has developed a variety of fluorescent based High- Content Assays with a microplate format, enabling a rapid and quantitative readout suitable for High- Content Analysis.

Assay No	Name/Species	Endpoint	Cell Type	Price
CS-SCs	Canine Sertoli Cell	Viability/Cytotoxicity, Multiplexed High-Content analysis	Canine, Primary Sertoli Cell	Quote
MU-SCs	Mouse Sertoli Cell	Viability/Cytotoxicity, Multiplexed High-Content analysis	Mouse, Sertoli Cell Line	Quote
MU-LCs	Mouse Leydig Cell	Viability/Cytotoxicity, Multiplexed High-Content analysis	Mouse, Leydig Cell Line	Quote
MU-SPs	Mouse Spermotogonial Cell	Viability/Cytotoxicity, Multiplexed High-Content analysis	Mouse, Spermotogonial Cell	Quote
HU-SCs	Human Sertoli Cell	Viability/Cytotoxicity, Multiplexed High-Content analysis	Human Primary Sertoli Cells	Quote
HU-LCs	Human Leydig Cell	Viability/Cytotoxicity, Multiplexed High-Content analysis	Human primary Leydig Cells	Quote
3D-T®Repro_Primary Canine	Canine Organoid	Viability/Cytotoxicity, Multiplexed High-Content analysis	Canine Primary Sertoli Cell	Quote
3D-T®Repro_Primary Mouse	Mouse reproductive 3D model	Viability/Cytotoxicity, Multiplexed High-Content analysis	Mouse primary testicular cells	Quote
3D-T®Repro_Primary Rat	Rat reproductive primary cell 3D model	Multiplexed High-Content analysis	Rat primary testicular cells	Quote

Assay No

Name/Species

Endpoint

Cell Type

Price

CS-SCs

Canine Sertoli Cell