ReproTOX has developed an animal-free 3D testicular cell co-culture system (3D-T®Repro) from a combination of testicular cells. This model has gone through extensive biomarker and morphology testing to ensure that it reflects in vitro condition and provides affordable, humane, and efficient methods for reproductive and developmental toxicity screening.
An extensive panel of toxicological endpoints is assessed in multiple cell types and at multiple time points using high content screening (HCS). By combining these endpoints with exposure levels, the system delivers a sensitive and specific prediction of repro-toxicity. With ReproTOX, we are at the forefront of cell-based high throughput (HT) and pathway-based High Content Analysis (HCA) for Reproductive-toxicity evaluations. This means we can provide a fully automated High Throughput Screen and High Content analysis as well as access to the reproductive toxicity database developed by ReproTOX (150 compounds).
3D-T®Repro_EX-Vivo provides an innovative technology to culture
seminiferous tubes, enable the spermatogenesis in vitro.
The cells recapitulate many features of spermatogenesis in
vivo, and enable assessment of differentiation and mitosis.
The technology preserves the original structure of the
seminiferous tubules, and the Sertoli cells remain polarized which
is crucial for the differentiation of germ cells. Thus, it allows
the study of both spermatogenesis in mitotic and meiotic phases.
This technology provides a reliable, robust, and relevant in
vitro system for preclinical drug discovery,
repro-toxicity assessment, and disease modeling.
Cytotoxicity screening panel
Mechanistic Toxicology Studies
Mitochondrial membrane potential
Functional mitochondrial toxicity
Cell viability (MTT, LDH and neutral red)
Cytoskelontal f-actin and tubulin alteration
DNA synthesis, M phase & in situ cell cycle analysis
Autophagy LC3B and LAMP
DNA damage early marker gammaH2Ax
Early or late apoptosis (caspase 3/7 or caspase 8/9)
Toxicological gene regulation
Androgen receptor modulation
Micronucleus test in germ cells
Custom Toxicology Service
Early assessment of drug safety
Proof of concept for drug mechanism of action or efficacy