ReproTOX has developed an animal-free 3D testicular cell co-culture system (3D-T®Repro) from a combination of testicular cells. This model has gone through extensive biomarker and morphology testing to ensure that it reflects in vitro condition and provides affordable, humane, and efficient methods for reproductive and development toxicity screening.

3D-T®Repro_Premier

An extensive panel of toxicological endpoints are assessed in multiple cell types and at multiple time points using high content screening (HCS). By combining these endpoints with exposure levels, the system delivers a sensitive and specific prediction of repro-toxicity. With ReproTOX, we are at the forefront of cell-based high throughput (HT) and pathway-based High Content Analysis (HCA) for Reproductive-toxicity evaluations. This means we can provide a fully automated High Throughput Screen and High Content analysis as well as access to the Reproductive toxicity Database developed by ReproTOX (150 compounds).


3D-T®Repro_EX-Vivo (in vitro spermatogenesis)

3D-T®Repro_EX-Vivo provides an innovative technology to culture seminiferous tubes, enable the spermatogenesis in vitro. The cells recapitulate many features of spermatogenesis in vivo, and enable assessment of differentiation and mitosis. The technology preserves the original structure of the seminiferous tubules, and the Sertoli cells remain polarized which is crucial for the differentiation of germ cells. Thus, it allows the study of both spermatogenesis in mitotic and meiotic phases. This technology provides a reliable, robust and relevant in vitro system for preclinical drug discovery, repro-toxicity assessment and disease modeling.

Examples of Service

Cytotoxicity screening panel

Multi-parametric Toxicity

Mechanistic Toxicology Studies

  Lysosomal trapping:LysoTracker

  Mitochondrial membrane potential

  Functional mitochondrial toxicity

  Cell viability (MTT, LDH and neutral red)

  Cytoskelontal f-actin and tubulin alteration

  DNA synthesis, M phase & in situ cell cycle analysis

  Autophagy LC3B and LAMP

  DNA damage early marker gammaH2Ax

  Early or late apoptosis (caspase 3/7 or caspase 8/9)

  Toxicological gene regulation

  Androgen receptor modulation

  Micronucleus test in germ cells

Custom Toxicology Service

  Early assessment of drug safety

  Proof of concept for drug mechanism of action or efficacy